What is the method to obtain a subset of a Seurat object using metadata as a criterion?

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To obtain a subset of a Seurat object using metadata as a criterion, you can use the subset function with the cells parameter.

For example, if you have a Seurat object called pbmc, and you want to obtain a subset of cells that have a metadata called stimulation with a value of IL2, you can use the following code:

subset_pbmc <- subset(pbmc, cells = WhichCells(pbmc, metadata = "stimulation", values = "IL2"))

In this code, WhichCells is used to obtain the cell indices that have the desired metadata and value, and these indices are used to subset the Seurat object. The resulting subset is stored in a new Seurat object called subset_pbmc.