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To decode a DataMatrix that includes a GS1 separator using Python, you can use a library such as pylibdmtx or OpenCV. These libraries provide functions to read and decode DataMatrix codes.

Here is an example using pylibdmtx:

import dmtx
from PIL import Image

# Load image of DataMatrix with GS1 separator
img = Image.open('datamatrix.png')

# Decode DataMatrix using pylibdmtx
data = dmtx.decode(img)

# Print decoded data 
print(data[0].data.decode())

In this example, we load an image of a DataMatrix code with a GS1 separator (saved as "datamatrix.png"). We then use the dmtx.decode() function to decode the code and store the result in a variable called "data". Finally, we print the decoded data by calling the data[0].data.decode() method.

Note: Make sure to install the required libraries before running the code.