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Yes, Firebase Authentication can be used with cross-origin isolation without the requirement of Firebase Hosting. Firebase Authentication is primarily used for authentication and authentication-related services, while cross-origin isolation is a browser security feature that prevents untrusted scripts from accessing sensitive information from other origins. To use Firebase Authentication with cross-origin isolation, you will need to enable the feature in your application and set the appropriate headers. This can be done regardless of whether your application is hosted on Firebase Hosting or not.