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You can remove the padding between items in a ListView by setting the padding property of the ListView to EdgeInsets.zero and the itemExtent property to the desired item height. Here's an example:

ListView.builder(
  padding: EdgeInsets.zero,
  itemExtent: 50,
  itemCount: items.length,
  itemBuilder: (context, index) {
    return ListTile(
      title: Text(items[index]),
    );
  },
);

Note that setting the itemExtent property can improve the performance of your ListView, but it may not be suitable for dynamic or variable height items. In that case, you can use a SliverList inside a CustomScrollView with a SliverPadding instead.