Is it impossible for the Spring Boot datasource to establish a connection with an MSSQL database when the user is registered in Active Directory?

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No, it is not impossible for Spring Boot datasource to establish a connection with an MSSQL database when the user is registered in Active Directory. However, the database configuration and authentication settings will need to be correctly set up to allow the connection to be made. The user's Active Directory credentials may also need to be correctly configured and granted appropriate database access permissions.